Senedd members debated Eluned Morgan's record following her first 100 days, with the first minister rejecting claims she has failed to stand up for Wales.
Andrew RT Davies led a Conservative debate on the eve of Thursday, which marks Eluned Morgan's hundredth day in office.
He accused the first minister of letting the country down, pointing to the withdrawal of the universal winter fuel allowance for pensioners and warnings of 4,000 premature deaths.
The leader of the opposition also criticised Labour's decision to raise national insurance contributions for employers, with unemployment in Wales at 5.3% and rising.
Mr Davies said 4,000 patients have been added to NHS waiting lists since the first minister took office in August, with a total of 614,000 people now waiting for treatment.
He told the Senedd: "That is a damning indictment of government failure here .... That is not standing up for patients here in Wales, it's not standing up for clinicians, and it's not standing up, importantly, for the workforce."
'Diktat'
Rhun ap Iorwerth said Baroness Morgan's first 100 days have shown little evidence of a change in direction from the Welsh Government.
The Plaid Cymru leader said: "By any objective measure, nothing has fundamentally changed in those 100 days."
He said Baroness Morgan has no plan to grow the economy nor tackle a crisis in the NHS.
Mr ap Iorwerth accused the first minister of failing to make the case for replacing the Barnett formula, devolving the Crown Estate, and compensating Wales for HS2 spending.
He said: "I'm afraid that what we've seen is Labour in Welsh Government, under the new first minister, shifting into the mode of defending their masters at Westminster...."
"A fundamental difference between Plaid Cymru and Labour is that we will never let Westminster diktat hamper our ambitions for Wales."
'Warmth'
Labour's Hefin David was unconvinced by the 100-day measure of success, which was coined by former US president Franklin Roosevelt in the 1930s.
He said: "It worked for him; I'm not sure it's going to work so much across modern politics, which moves so quickly and so differently."
He suggested the next Senedd election in 18 months will be a much better yardstick.
The Caerphilly Senedd Member pointed out that Wales' first female first minister, from Ely, Cardiff, one of the poorest parts of the UK, succeeded against the odds.
Describing Baroness Morgan as a "listening first minister", Dr David said: "She's the only first minister who gives me a cwtch every time I see her. I can see Mark Drakeford getting a little worried there. I'm not expecting anything, finance minister."
"But I do think it demonstrates the warmth of Eluned Morgan."
'Lengthly list'
Responding to the debate, Eluned Morgan reeled off a list of achievements including £28m to cut waiting times, £13m on better end of life care and a new North Wales medical school at Bangor University.
She said £7.7 million has been invested in a specialist burns and plastic surgery centre at Swansea's Morriston Hospital, serving ten million people from Aberystwyth to Oxford.
Baroness Morgan lauded a "landmark" £1 billion investment in the redevelopment of Shotton Mill, Deeside, protecting 137 jobs and creating 220 more.
She claimed the Labour Welsh and UK Governments also secured a better deal for Tata steelworkers, accusing the Tories of failing to budget for a £80m transition fund.
"This is a lengthy list," she said. "But it could be longer and it will be longer as we continue to deliver....the first 100 days demonstrates how Welsh Labour is delivering real investment, real jobs, real support for communities – not promises and pledges but delivery.
"I am so proud of everything this government has already delivered since I became first minister and I'm optimistic about what we can achieve as we move forward."
Footpath row: couple seek High Court action
Man dies at Ogmore-by-Sea
Dinas Powys street on 'most expensive' list
Minister serves up free Christmas school meals
Accreditation for Barry Hospital ward
Burnett on homelessness, housing and budget pressures
Meet the Gavin and Stacey home owner who has welcomed almost 30,000 people into her home
Over £30k raised for Santa's Cause appeal
Sports clubs secure energy saving funding
Christmas parties held for Vale families
Youth charged after Barry stabbing
Councillors back Lidl Llantwit plans
Council sets out Christmas recycling plans
Panto stars visit Marie Curie hospice
Gavin and Stacey - the puppies heading home for Christmas
Gavin and Stacey star raffles off signed Finale Script
Christmas pop-up sauna returns to Penarth
Senedd 'super constituency' plans redrawn
