Llantwit Major have applied for an FAW licence to play in the Cymru Premier.
The Cymru South leaders are among 26 Welsh clubs who have stated their interest in a Tier One licence for the 2022-23 season.
All twelve Cymru Premier teams - including Barry Town United - have also applied, along with a further seven Tier Two clubs in the South.
The Tier One licence is necessary for playing in the top flight of Welsh domestic football.
Besides their on-pitch fortunes, clubs have to meet stringent criteria from league officials, such as youth and coaching facilities, infrastructure and financial matters.
Llantwit Major are among three teams applying for a top-tier licence for the very first time.
Having emerged as surprise frontrunners, Karl Lewis' side remain unbeaten in the Cymru South after ten games and have swept the league's monthly awards for August and September.
In the past six years, the Windmill Lane club has moved up three steps in the Welsh domestic pyramid. Under Lewis, they were crowned champions in Divisions Two and Three of the former Welsh Football League.
Two years ago, Major were among the founder members of the Cymru South, as part of a wider restructure of the men's leagues in Wales.
After the inaugural season was curtailed by COVID, Swansea University were declared champions on a points-per game basis - but they were denied a Tier One licence. Instead, runners-up Haverfordwest County were promoted.
The FAW says: ''The club licensing system aims to assist the continual improvement and development of football clubs at the top level within Wales.''
Between now and the end of the year, FAW officials will carry out club audits by visiting their grounds and assessing their licence documents.
In all, there are five key areas on which the licences are decided:
Prospective clubs must also play at grounds that can hold at least 1500 spectators, including 500 covered seats for Cymru Premier and league cup matches.
The applications must be finalised by the end of March 2022, before a licensing panel meets in Cardiff to make its decision in April. If clubs are refused a Tier One licence, they can lodge an appeal, which will be heard a fortnight later.
The twelve Cymru Premier teams, who have applied for a Tier One licence for next season, are:
The eight Cymru South (tier two) clubs who have applied are:
Notably, reigning Cymru South champions Swansea University have decided not to apply this time around - while only Carmarthen and Llanelli have past experience playing in the Cymru Premier.